| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Staunton | A | Matesa | Ogata | Haas | Rantanen | 3:59.10 |
| 2 | Wood River (East Alton-W.R.) | A | Wilcutt | Loeffelman | Gould | Marshall | 3:55.00 |
| 3 | Trenton (Wesclin) | A | Evans | Kapp | Hendrix | Waggoner | 3:51.00 |
| 4 | Freeburg | A | Walters | Schottel | Hoover | Mense | 4:20.00 |
| 5 | East St. Louis (SIUE Charter | A | Foster | Foster | Anthony | Ray | DNS |
| 6 | Waterloo (Gibault Catholic) | A | Biffar | Nevois | Stern | Matthews | 3:52.30 |
| 7 | Breese (Mater Dei) | A | Diekemper | Holthaus | Goring | Shelton | 3:55.00 |