| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Madison | A | Gregory | Rosser | Belford | Griggs | NT |
| 4 | Freeburg | A | Atchison | Varty | Evans | Thompson | 1:00.00 |
| 5 | Nokomis | A | Watson | Kirk | Carroll | Waterman | 1:00.71 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Staunton | A | Daugherty | Trettenero | Fulton | Kilduff | DNS |
| 2 | Breese (Mater Dei) | A | Becker | Kampwerth | Strubhart | Zurliene | 56.50 |
| 3 | Trenton (Wesclin) | A | Roberts | Bernreuter | Marks | Evans | 54.80 |
| 4 | Alton (Marquette) | A | Bryson | Stephenson | Wells | Rae | 53.30 |
| 5 | Carrollton | A | Darr | Field | Flowers | Brannan | 53.50 |
| 6 | Wood River (East Alton-W.R.) | A | Brown | Minner | Nguyen | Truax | 55.55 |
| 7 | East St. Louis (SIUE Charter | A | Black | Leech | Parker | Spiller | DNS |