| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Freeburg | A | Zhu | Young | Byman | Huelsmann | 1:50.00 |
| 4 | Carrollton | A | Cox | Gilmore | Harrelson | Settles | DNS |
| 5 | Trenton (Wesclin) | A | Waggoner | Lopez | Bateman | Evans | 1:47.10 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Wood River (East Alton-W.R.) | A | Skinner | Lane | Marshall | Gould | 1:41.10 |
| 3 | East St. Louis (SIUE Charter | A | Foster | Foster | Ray | Spiller | 1:40.64 |
| 4 | Madison | A | Moore | Williams | Rogers | Trammel | 1:34.20 |
| 5 | Breese (Mater Dei) | A | Goring | Rakers | Grapperhaus | Jourdan | DNS |
| 6 | Staunton | A | Vercoglio | Rantanen | Haas | Trettenero | 1:40.80 |
| 7 | Nokomis | A | Altevogt | Blaum | Johnson | Houston | 1:43.21 |