| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | East St. Louis (SIUE Charter | A | Black | Leech | Spiller | Parker | DNS |
| 2 | Freeburg | A | Vahlkamp | Atchison | Evans | Thompson | 2:10.00 |
| 3 | Wood River (East Alton-W.R.) | A | Brown | Minner | LeGette | Sanders | 2:04.50 |
| 4 | Alton (Marquette) | A | Bryson | Stephenson | Wells | Rae | 2:00.00 |
| 5 | Trenton (Wesclin) | A | Roberts | Marks | Waggoner | Lopez | 2:00.40 |
| 6 | Staunton | A | Fulton | Kilduff | Daugherty | Trettenero | 2:06.00 |
| 7 | Madison | A | Gregory | Rosser | Belford | Coulibaly | NT |