| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Collinsville | A | Crowder | Holmes | Tillman | Skertich | 52.00 |
| 2 | Belleville West | A | Rockett | Chapman | Singleton | Melvin | 49.80 |
| 3 | Edwardsville | A | Thompson | Carr | Hockett | Sanders | DQ |
| 4 | Belleville East | A | Robinson | Bacon | Chism | Durrah | 48.52 |
| 5 | O'Fallon | A | Clark | Daniels | Pelly | Perry-Anderson | 48.83 |
| 6 | Alton | A | Raglin | Farris | Carter | Ingram | 49.00 |
| 7 | Bloomington | A | Phillips | Witt | Ifft | Boombo | 50.23 |
| 8 | Granite City | A | Thomas | Kiump | Posten | Moss | 52.68 |