| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Olympia | A | Gellner | Alexander | Ragar | Sitton | 48.00 |
| 2 | Hillsboro | A | White | Holcomb | Hamby | Anderson | 47.66 |
| 3 | Highland | A | Nimmo | Ward | Story | Fridley | 47.11 |
| 4 | Breese Central | A | Shubert | Revermann | Attaway | Rogers | 46.60 |
| 5 | Wesclin | A | Hendrix | Evans | Kapp | Monical | 46.90 |
| 6 | Metro-East Lutheran | A | Fedder | Burnett | Chernianskii | Kohlmiller | 47.50 |
| 7 | Vandalia | A | Keck | Whitehead | Wilkerson | Gencel | 47.80 |
| 8 | Dupo | A | Schroeder | Prater | Jordan | Jordan | 49.50 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Mattoon | A | Arnold | Mosley | Karpus | Kimbro | 46.24 |
| 2 | Jersey | A | Withrow | Talley | Borkowski | Jones | 45.60 |
| 3 | Freeburg | A | Murphy | Jarvis | Merz | Thompson | 45.35 |
| 4 | Olney (Richland County) | A | Jones | Pianfetti | Runyon | Cothern | 44.20 |
| 5 | Rochester | A | Pletsch | Slott | Huet | Barton | 45.00 |
| 6 | Columbia | A | Scott | Nelson | Donavan | Kronk | 45.40 |
| 7 | Greenville | A | Green | File | Redding | Baum | 45.72 |
| 8 | Pana | A | Carlson | Sims | Lynch | Beyers | 46.50 |