| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Dupo | A | Schroeder | Reyes | Bean | Ross | 4:39.80 |
| 4 | Wesclin | A | VanGorkom | Haas | Waggoner | Gruenke | 4:04.90 |
| 5 | Vandalia | A | Peters | Flowers | Jones | Culpepper | 4:13.80 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Metro-East Lutheran | A | Pryor | Goeckner | Wyatt | Schlessinger | 3:58.00 |
| 3 | Hillsboro | A | Hubbart | Fowler | Hamby | Wells | 3:52.00 |
| 4 | Rochester | A | Huet | Bruce | Mrozowski | Hobson | 3:49.00 |
| 5 | Mattoon | A | Kirts | Gordon | Moore | Karpus | 3:50.10 |
| 6 | Jersey | A | Judkins | Little | Burch | Kribs | 3:57.00 |
| 7 | Pana | A | Elledge | Carlson | Kile | Harbert | 3:59.83 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Olney (Richland County) | A | Brown | Zwilling | Kirby | Jones | 3:45.00 |
| 2 | Columbia | A | Scott | Donavan | Mueth | Hogan | 3:43.00 |
| 3 | Freeburg | A | Duffie | Pluff | Banks | Frech | 3:41.14 |
| 4 | Highland | A | Fridley | Mancinas | Basden | Smith | 3:40.00 |
| 5 | Breese Central | A | Hunt | Jansen | Becker | Attaway | 3:40.10 |
| 6 | Olympia | A | Alexander | Sitton | Demling | Uphoff | 3:42.00 |
| 7 | Carlinville | A | Sproull | Dunn | Roper | Rouse | 3:45.00 |
| 8 | Greenville | A | Redding | File | Bohn | Battaglia | 3:46.57 |