| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Carlinville | A | Jackson | Whaley | Widner | Wiese | 59.20 |
| 2 | Rochester | A | Browning | Davis-Hill | Johnson | Bruzon-Lobaton | 57.60 |
| 3 | Vandalia | A | Swyers | Duplayee | McNealy | Rush | 56.62 |
| 4 | Mattoon | A | Hettinger | Bradley | Gouveia | Barnes | 56.00 |
| 5 | Hillsboro | A | Hughes | Christian | Frailey | Rupert | 56.60 |
| 6 | Pana | A | Edwards | Woolard | Beyers | Snyder | 57.12 |
| 7 | Jersey | A | Leslie | Soffray | Weber | Deist | 58.60 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Olympia | A | Downs | Bieber | Miller | Whiteside | 56.00 |
| 2 | Greenville | A | Bellegante | Rohr | Neely | DeBlois | 54.70 |
| 3 | Breese Central | A | Morris | Taylor | Rakers | Bassler | 54.01 |
| 4 | Pleasant Plains | A | Bergner | Pearce | Weber | Morley | 53.00 |
| 5 | Freeburg | A | Schulz | Nissen | Stroder | Hurst | 53.52 |
| 6 | Dupo | A | Jordan | Thompson | Woods | Latimer | 54.10 |
| 7 | Olney (Richland County) | A | Gibson | Travis | Bahl | Clodfelter | 55.00 |
| 8 | Highland | A | Choat | Pollard | Thomas | Maas | 56.00 |