| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Salem | A | Malan | Purintun | Patterson | Salgado | 4:05.00 |
| 4 | Jersey | A | Judkins | Burch | Wilson | Little | 4:00.00 |
| 5 | Civic Memorial | A | Gares | Flanagan | Zampieri | Kearbey | 4:05.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Effingham | A | Spicer | Flack | Gordon | Hill | 3:56.00 |
| 2 | Mascoutah | A | Lara | Clark | Williams | Perryman | 3:54.62 |
| 3 | Edwardsville | A | Kitchen | Bruhn | Malone | Arendell | 3:48.18 |
| 4 | O'Fallon | A | Wocking | Ross | Sheppard | Lewis | 3:43.00 |
| 5 | Triad | A | Dempsey | Vanzandt | Harmon | Twyman | 3:48.00 |
| 6 | Collinsville | A | Strong | Patterson | Shelton | Taylor | 3:50.00 |
| 7 | Highland | A | Smart | Knobloch | Miller | Parsons | 3:55.00 |
| 8 | Father McGivney Catholic | A | Ahring | Boeving | Schmidt | Biermann | 4:00.00 |