| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Normandy | A | Rhodes | Sullivan | Moore | Piphus | 1:39.00 |
| 3 | Vashon | A | Phillips | Taylor | Aaron | Blockett | 1:37.50 |
| 4 | SIUE Charter | A | Foster | Spiller | Wells | Shaw | 1:35.65 |
| 5 | Hazelwood West | A | Anderson | Johnson | Webster | Johnson | 1:33.20 |
| 6 | Belleville West | A | Jackson | Mcclean | Nolan | Fountian | 1:34.00 |
| 7 | East St. Louis | A | Smith | Ryan | Lawary | Avan | 1:36.00 |
| 8 | Marian Catholic (C.H.) | A | Holland | Griffin | Key | Frazier | 1:38.50 |
| 9 | Manual | A | Postlewaite Jr. | Filzen | Dailey | White | 1:44.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Kirkwood | A | Welch | Edwards | Webb | Richardson | 1:33.00 |
| 2 | Westminster Christian | A | Avery | Heard | Lee | Lowry | 1:32.58 |
| 3 | Kenwood | A | Smith | Trotter | Moreno | Washington | 1:31.46 |
| 4 | Cahokia | A | Baxtron | Johnson-Cutler | Moore | Howard | 1:30.90 |
| 5 | Riverview Gardens | A | Howard | Abel | Anderson | Thompson | 1:29.00 |
| 6 | Cardinal Ritter | A | Boyd | Saddler | Winner-Johnson | Steed-Allen | 1:30.84 |
| 7 | Hazelwood Central | A | Johnson | Anthony | Martin | Williams | 1:31.07 |
| 8 | Madison | A | Boyd | Bursey | Wilson Jr. | Moore | 1:32.10 |
| 9 | St. Laurence | A | Rizzs | Potter | Enoch | Hannah lll | 1:32.71 |