| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 4 | Father McGivney Catholic | A | Deist | Welser | Shearer | Joellenbeck | 1:53.00 |
| 5 | Althoff Catholic | A | Nave | Cole | Jackson | Johaningmeyer | 1:45.00 |
| 6 | East Alton-Wood River | A | Burgess Jr | Marshall | Lane | Skinner | 1:45.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Civic Memorial | A | Wooten | Fonrodona | Silva | Gares | 1:45.00 |
| 3 | Hillsboro | A | Fowler | Clayton | Anderson | Lessman | 1:44.54 |
| 4 | Highland | A | Fridley | Nimmo | Schonhardt | Ward | 1:44.04 |
| 5 | Vandalia | A | Keck | Gencel | Wilkerson | Whitehead | 1:43.59 |
| 6 | Glenwood | A | Keppler | Antonacci | Massey | Flatt | 1:44.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Quincy | A | Brockmiller | O'Hara | Schenk | Hammers | 1:43.00 |
| 2 | Litchfield | A | Jones | Grammer | Leitschuh | Powell | 1:42.65 |
| 3 | Rochester | A | Bruce | Mrozowski | Olson | Pletsch | 1:42.50 |
| 4 | Red Bud | A | Dressel | Baldwin | Schwartzkopf | Wagner | 1:42.40 |
| 5 | Jersey | A | Borkowski | Loges | Talley | Jones | 1:42.00 |
| 6 | Freeburg | A | Merz | Jarvis | Loftus | Zhu | 1:42.13 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Staunton | A | Trettenero | Rantanen | Matesa | Buffington | 1:41.00 |
| 2 | Auburn | A | Sharp | Piper | Lancaster | Kern | 1:40.83 |
| 3 | Waterloo | A | Brewer | Huebner | Pete | Kettler | 1:39.00 |
| 4 | Granite City | A | Brokaw | Rodgers | Webb | Dean | 1:37.53 |
| 5 | Mascoutah | A | Owens | Feldt | Phillips | Killian | 1:36.68 |
| 6 | Roxana | A | Coles | Wieneke | Ellis | McBride | 1:36.95 |