| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Waterloo | A | Stratton | Schimpf | Schwartz | Corey | 8:30.00 |
| 2 | Mascoutah | A | Moll | Drain | Cozzi | Fuess | 8:39.09 |
| 3 | Freeburg | A | LaChance | Schlichting | Rozhon | Duffie | 9:00.00 |
| 4 | Highland | A | Rednour | Wakeley | Smart | Roach | 9:05.66 |
| 5 | Rochester | A | Hardy | Oliver | Armbruster | Hardy | 9:12.58 |
| 6 | Roxana | A | Ponce | McVey | Hardin | Pride | 9:20.00 |
| 7 | Civic Memorial | A | Zampieri | Kearby | Walker | Runyan | DNF |
| 8 | East Alton-Wood River | A | Wilcutt | Simpson-Kolmer | Baker | Jones | 9:30.10 |
| 9 | Red Bud | A | Wagner | Schluter | Grohmann | Hill | 9:42.15 |
| 10 | Quincy | A | Steinkamp | Anders | Lithila | Steiner | 9:46.80 |
| 11 | Jersey | A | Little | Burch | Turnbaugh | Wade | 9:50.00 |
| 12 | Father McGivney Catholic | A | Schmidt | Schmidt | Huber | Shearer | 9:50.00 |
| 13 | Althoff Catholic | A | Schillinger | Tell | Schallert | Telafuse | 9:54.35 |
| 14 | Litchfield | A | Floyd | Schwab | Maguire | Quarton | 9:58.64 |
| 15 | Glenwood | A | House | Hammon | Bostrom | Iwanicki | 10:15.00 |
| 16 | First Baptist Academy | A | Clydesdale | Devine | Miller | Lipe | 10:30.00 |