| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | East Alton-Wood River | A | Minner | Sanders | Nguyen | Truax | DNS | |
| 3 | Alton Marquette | A | Davenport | Johnson | Wells | Certa | 4:45.00 | |
| 4 | Father McGivney Catholic | A | Hatley | Cummins | Rakers | Rybak | 4:17.00 | |
| 5 | Civic Memorial | A | Hannaford | Vaughn | Mann | Meiser | 4:28.00 | |
| 6 | Roxana | A | Kimbro | Doyle | Losch | Partridge | 5:02.27 |