| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 4 | Civic Memorial | A | Crymes | Shields | Govero | Brousseau | 55.52 |
| 5 | Freeburg | A | Schulz | Nissen | Range | Hurst | 55.82 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Nashville | A | Thorson | Matecki | Vogt | Newman | 53.58 |
| 2 | Okawville | A | Luechtefeld | Statler | Haacke | Velazquez-Reyes | 53.21 |
| 3 | Highland | A | Henschen | Pollard | Thomas | Maas | 53.00 |
| 4 | Breese Central | A | Morris | Taylor | Rakers | Bassler | 52.63 |
| 5 | Granite City | A | Thomas | Brown | Posten | Lee | 52.68 |
| 6 | Carbondale | A | Penn | Hamilton | Harris | Santano | 53.18 |
| 7 | Waterloo | A | Zweigart | Springer | Barringer | Starek | 53.40 |
| 8 | Salem | A | Puricelli | Cape | Alli | Robinson | 55.26 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Triad | A | Cissell | Witham | Brown | Schardan | 51.71 |
| 2 | Mascoutah | A | Lowe | Love | Athy | Schneider | 51.10 |
| 3 | Mt. Vernon | A | Johnson | Sneddon | Delatore | Pollard | 50.22 |
| 4 | O'Fallon | A | Perry-Anderson | Daniels | Clark | Pelly | 48.98 |
| 5 | Cahokia | A | Austin | Hayes | Tucker | Davis | 50.00 |
| 6 | Collinsville | A | Clemons | Gary | Johnson | Spears | 50.97 |
| 7 | Marion | A | Greer | Kelly | Ankrom | Williams | 51.50 |
| 8 | Madison | A | Gregory | Kelly | Belford | Rosser | FS |