| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Salem | A | Cape | Pizarro | Donoho | Jarrett | 2:04.71 |
| 4 | Okawville | A | Velazquez-Reyes | Meier | Cochran | Wolf | 1:59.40 |
| 5 | Civic Memorial | A | Coffman | Anderson | wehmeier | Crymes | 2:04.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Mater Dei | A | Zurliene | Kampwerth | Strubhart | Becker | 1:58.80 |
| 2 | Triad | A | Nadler | Witham | DiRosa | Brown | 1:55.00 |
| 3 | Carbondale | A | Penn | Harris | Collier | Hamilton | 1:54.14 |
| 4 | Madison | A | Kelly | Rosser | Couliday | Gregory | 1:53.00 |
| 5 | Nashville | A | Thorson | Matecki | Harris | Newman | 1:53.58 |
| 6 | Breese Central | A | Tebbe | Detmer | Taylor | Luth | 1:54.45 |
| 7 | Collinsville | A | Skertich | Briggs | Holmes | Crowder | 1:56.00 |
| 8 | Freeburg | A | Schulz | Nissen | Dircks | Hurst | 1:58.82 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Waterloo | A | Zweigart | Springer | Barringer | Prather | 1:52.85 |
| 2 | Marion | A | Greer | Stearns | Kelly | Williams | 1:49.04 |
| 3 | Mascoutah | A | Lowe | Love | Waddell | Schneider | 1:48.00 |
| 4 | Granite City | A | Miller-Brown | Posten | Henry | Fenoglio | 1:45.78 |
| 5 | O'Fallon | A | Daniels | Perry-Anderson | Clark | Prindable | 1:46.08 |
| 6 | Mt. Vernon | A | Johnson | Sneddon | Delatore | Pollard | 1:48.20 |
| 7 | Cahokia | A | Ransom | Lyons | Brooks | Pomerlee | 1:50.50 |
| 8 | Highland | A | Henschen | Pollard | Maas | Thomas | 1:53.00 |