| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Carbondale | A | Buffington | Santano | Ekpe | Miller | 4:45.00 |
| 2 | Mater Dei | A | Becker | Strubhart | Kampwerth | Faus | 4:34.00 |
| 3 | Civic Memorial | A | Hannaford | Vaughn | Mann | Meiser | 4:28.00 |
| 4 | Marion | A | Odum | Stearns | Ankrom | Kelly | 4:20.00 |
| 5 | Breese Central | A | Morris | Rakers | Tebbe | Detmer | 4:27.10 |
| 6 | Mt. Vernon | A | Johnson | Newell | Sneddon | Delatore | 4:31.63 |
| 7 | Granite City | A | Bonner | Franklin | Harris | Fenoglio | 4:35.28 |
| 8 | Highland | A | Friederich | Keeney | Peters | Maas | 4:50.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Collinsville | A | Hawkes | Gary | Clemons | Spears | 4:20.00 |
| 2 | Salem | A | Laird | Krajefska | Alli | Puricelli | 4:57.55 |
| 3 | O'Fallon | A | Prindable | Parker | Perry-Anderson | Knapp | 4:17.00 |
| 4 | Waterloo | A | Thorsten | Smith | Starek | Grohmann | 4:08.35 |
| 5 | Freeburg | A | Trentman | Geluck | Trentman | Harmon | 4:12.79 |
| 6 | Nashville | A | Thorson | Schmale | Wilson | Newman | 4:18.00 |
| 7 | Cahokia | A | Tucker | Millsap | Moncelli | Brooks | 4:20.00 |
| 8 | Mascoutah | A | Drain | Creamer | Maschhoff | Kimmle | 4:20.00 |