| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | O'fallon Fulton MS | A | Batten | Rivet | Bramer | Kloever | NT |
| 3 | Highland MD | A | Staley | Rottmann | Renko | Reckmann | 59.00 |
| 4 | Alton Middle School | A | Lucas | Williams | Sutherlin | Evans | 53.70 |
| 5 | Mascoutah Middle School | A | Thompkins | Stefanik | Ross | Kruzelnick | 54.04 |
| 6 | Amelia V. Carriel Junior High | A | Burgess | Patterson | Dallner | Woodhouse | NT |
| 7 | Dupo Tigers | A | Johnson | Chartrand | Byrd | Thornton | NT |