| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Amelia V. Carriel Junior High | A | Woodhouse | Juenger | Burgess | Gentille | NT |
| 3 | Dupo Tigers | A | Byrd | Hoffman | McGlasson | Thornton | NT |
| 4 | Alton Middle School | A | Murphy | Williams | Hudson | Kemper | 1:15.00 |
| 5 | Highland MD | A | Lindsey | Ellis | Fenton | Lindsey | 1:36.00 |
| 6 | Mascoutah Middle School | A | Finlay | Evans | Taylor | Garland | NT |