| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Mascoutah Middle School | A | Hawkins | Hendershot | Schneider | Rankin | NT |
| 2 | Carriel Junior High | A | Lee | Bridges | Steen | Whitfield | NT |
| 3 | Highland MD | A | Hadowsky | Adoplh | Hadowsky | Klaus | 1:53.10 |
| 4 | Lincoln Middle School | A | Pelly | Conner | Jackson | Samuels | 1:43.00 |
| 5 | Alton Middle School | A | Harley | Gibson | Poindexter | Murrell | 1:44.90 |
| 6 | Whiteside Middle School | A | Donaldson | Henderson | Taylor | Fequierre | NT |
| 7 | Dupo Tigers | A | Finley | Johnson | Putnam | Portell | NT |
| 8 | O'fallon Fulton MS | A | Daniel | Whitten | Coffee | Allen | NT |