| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | Grace COLGAN | SO | Freeburg | 56.94 | |
| 3 | Jazmine MONCELLI | FR | Cahokia | 53.12 | |
| 4 | Imani CHAPMAN | SR | Belleville West | 51.80 | |
| 5 | Natalie DRAIN | JR | Mascoutah | 50.99 | |
| 6 | Jamison LOVE | SO | Mascoutah | 51.17 | |
| 7 | Skylar WOODS | FR | East St. Louis | 52.30 | |
| 8 | Monica PARKER | JR | O'Fallon | 53.20 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | Kyla CASON | SR | O'Fallon | 50.80 | |
| 3 | Zariah ARMOUR | SO | Belleville East | 49.68 | |
| 4 | Andrianna MILLSAP | SR | Cahokia | 48.15 | |
| 5 | JaKariah BACON | SO | Belleville East | 47.05 | |
| 6 | Da'Wyriah BLANCHARD | SO | Belleville West | 47.78 | |
| 7 | AnnMarie TRENTMAN | FR | Freeburg | 48.15 | |
| 8 | Jaylah HARRIS | FR | East St. Louis | 50.07 |