| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Collinsville | A | Clemons | Gilmore | Jeremias | Spears | 50.82 |
| 2 | Alton | A | Farris | Raglin | Ingram | Steen | 49.90 |
| 3 | East St. Louis | A | McClain | Jones | Graham | Glenn | 48.47 |
| 4 | O'Fallon | A | Clark | Daniels | Perry-Anderson | Tart | 47.96 |
| 5 | Belleville East | A | Chism | Armour | Bacon | Robinson | 48.23 |
| 6 | Belleville West | A | Rockett | Singleton | Sparks | Melvin | 49.80 |
| 7 | Edwardsville | A | Sanders | Thompson | Barton | Carr | 49.97 |