| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Edwardsville | A | Fryer | Davis | Bollinger | Samet | 4:35.00 |
| 3 | O'Fallon | A | Simmons | Johnson | Knapp | Newble | 4:18.00 |
| 4 | Belleville West | A | Blue | Byrum | Brown | Thomas | 4:03.80 |
| 5 | East St. Louis | A | Simmons | Tugle | Askew | Taylor | 4:08.00 |
| 6 | Alton | A | Edwards | Rehling | Jones | Klockenkemper | DNS |