| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Mascoutah | A | Young | Carter | Waddell | Keely | DNF |
| 3 | O'Fallon | A | Daniels | Perry-Anderson | Pelly | Clark | 50.25 |
| 4 | Belleville West | A | Singleton | Sparks | Chapman | Lewis | DNF |
| 5 | Edwardsville | A | Thompson | Steinhauff | Sanders | Barton | 48.95 |
| 6 | Granite City | A | Miller-Brown | Kiump | Supinski | Fenoglio | DNS |
| 7 | Collinsville | A | Briggs | Crowder | Holmes | Skertich | 55.00 |