| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Mascoutah | A | Stoltz | France | Clark | Drain | 4:15.00 |
| 3 | Granite City | A | Rodgers | Walker | Dean | Wilson | DNF |
| 4 | Alton | A | Singleton | Taul | Whitfield | Kotzamanis | 3:45.00 |
| 5 | O'Fallon | A | Doss | Faver | Schram | Greenstreet | 3:45.00 |
| 6 | Edwardsville | A | Malone | Arendale | Wilkerson | Baxter | 3:55.00 |
| 7 | Maryville Christian | A | King | Malone | Ury | Jacob | 4:45.00 |