| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Granite City | A | Brokaw | Webb | Rodgers | Rodgers-Parott | 45.70 |
| 3 | Edwardsville | A | Cole | Range | Henderson | Word | 44.24 |
| 4 | Alton | A | Harris | Elliot-Barnes | Johnson | Taul | 43.50 |
| 5 | O'Fallon | A | Johnson | Dowell | Beverly | Mims | 44.20 |
| 6 | Mascoutah | A | Reed | France | Allen | Holcomb | 44.61 |