| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Edwardsville | A | Stemmons | Henderson | Heavens | Word | 1:38.00 |
| 3 | Mascoutah | A | Stoltz | Reed | Phillips | Holcomb | 1:36.74 |
| 4 | Alton | A | Singleton | Elliot-Barnes | Harris | Johnson | 1:33.00 |
| 5 | O'Fallon | A | Green | Beverly | Dowell | Mims | 1:35.00 |
| 6 | Granite City | A | Brokaw | Walker | Ford | Rodgers | 1:37.53 |
| 7 | Maryville Christian | A | Gilliland | Abert | King | Ury | 1:50.00 |