| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Collinsville | A | Holmes | Dodd | Hall | Hughes | DNS | |
| 2 | Madison | A | Gregory | Rosser | Kelly | Couliday | 2:05.00 | |
| 3 | O'Fallon | A | Simmons | Johnson | Prindable | Newble | 1:55.00 | |
| 4 | East St. Louis | A | Singleton | Harris | Falconer | Taylor | 1:50.45 | |
| 5 | Alton | A | Ingram | Harris | Jones | Raglin | 1:53.00 | |
| 6 | Edwardsville | A | Bell | Hockett | Carr | Flannery | 1:57.00 | |
| 7 | Belleville East | A | Saffold | Harris | Williams | Miller | 2:05.00 |