| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Edwardsville | A | Davis | Flannery | Cruzin | Arensmann | 53.00 | |
| 3 | Collinsville | A | Spears | Gilmore | Tillman | Skertich | DNS | |
| 4 | Alton | A | Harris | Jones | Raglin | Ingram | 51.00 | |
| 5 | East St. Louis | A | Singleton | Taylor | Gilliespie | Miller | 51.89 | |
| 6 | Madison | A | Gregory | Kelly | Compton | Rosser | 52.47 | |
| 7 | O'Fallon | A | Simmons | Johnson | Pinckney | Newble | DNF |