| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Madison | A | Couliday | Compton | Gregory | Rosser | 5:00.00 | |
| 2 | Belleville East | A | Wilson | Quarles | Peppers | Tolar | 4:35.00 | |
| 3 | O'Fallon | A | Knapp | Prindable | Simmons | Pinckney | 4:24.00 | |
| 4 | Alton | A | Miller | Ingram | Stark | Raglin | 4:18.00 | |
| 5 | East St. Louis | A | Askew | Tugle | Taylor | Simmons | 4:20.00 | |
| 6 | Collinsville | A | Hawkes | Dixon | Spears | Jimenez | DNS | |
| 7 | Edwardsville | A | Haislar | Davis | Samet | Bollinger | 4:44.00 |