| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Columbia | A | Habermehl | Acevado | Hogan | Range | 8:22.00 |
| 2 | East St. Louis | A | Ivy | Cross | Ellard | Sanders | 8:35.00 |
| 3 | Civic Memorial | A | Naugle | Dutton | Eldridge | Collman | 8:40.00 |
| 4 | St. Teresa | A | Kernaghan | Cook | Striglos | Kernaghan | 8:44.16 |
| 5 | Alton | A | Gallivan | Cowan | Kotzamanis | McAfoos | 8:47.00 |
| 6 | Mascoutah | A | Ellsworth | Moll | Latham | Fuess | 8:58.51 |
| 7 | Belleville East | A | Butler | Smith | Ziegler | Schuermann | 9:00.00 |
| 8 | Triad | A | Twyman | Pace | Mersinger | Metcalf | 9:00.00 |
| 9 | Pittsfield | A | Olson | Oitker | Veile | Veile | 9:10.00 |
| 10 | East Alton-Wood River | A | Wilcutt | Simpson-Kolmer | Baker | Loffleman | 9:30.10 |
| 11 | Greenville | A | Wilson | Rodgers | Williams | Siebert | 10:00.35 |
| 12 | Shelbyville | A | Schurict | Hilbert | Fisher | Anderson | 10:15.00 |
| 13 | Staunton | A | Monahan | Albertina | Myers | Best | 11:43.00 |