| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Marion | A | Robertson | Lange | Cooper | Murkel | 48.28 | |
| 3 | Civic Memorial | A | Flanagan | Slotta | Books | Gares | 46.80 | |
| 4 | Mt. Vernon | A | Lusby | Jefferson | Kessler | Knappier | 45.90 | |
| 5 | O'Fallon | A | Pasley | Clark | Fisher | Ross | 46.35 | |
| 6 | Collinsville | A | Harper | Patterson | Thomas | Mayne | 46.84 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Andrew | A | Eassa | Thigpen | Thompson | Danca | DQ | |
| 2 | Alton | A | Rounds | Johnson | Holeman | White | 45.41 | |
| 3 | Edwardsville | A | Cole | Wahl | Montgomery | 44.13 | ||
| 4 | Glenwood | A | Sweeney | Smith | Moomey | Ladage | 43.80 | |
| 5 | Plainfield North | A | Coleman | Cyracus | Bownes | Anderson | 44.06 | |
| 6 | Effingham | A | Buzzard | Lybarger | Wendling | Latham | 45.24 | |
| 7 | Highland | A | Wallace | Miller | Fridley | Nimmo | 45.45 | |
| 8 | Triad | A | Hard | Lightner | Coleman | Dempsey | 45.90 |