| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Civic Memorial | A | Silva | Wooten | Atteberry | Fonrodona | 46.80 | |
| 2 | Effingham | A | Freeman | Walls | Weaver | Dunsten | 45.24 | |
| 3 | Olney (Richland County) | A | Pianfetti | Cothern | Hallam | Cothern | 43.90 | |
| 4 | Marion | A | Wiseman | Wade | Thomas | Beverly | 43.73 | |
| 5 | Glenwood | A | Rayl | Marcordes | Sgro | Saunders | 43.80 | |
| 6 | Newton | A | Baltzell | Flowers | Zumbahlen | Baltzell | 43.98 | |
| 7 | Granite City | A | Brokaw | Dean | Rodgers | Rodgers-Parott | 45.42 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | O'Fallon | A | Johnson | Dowell | Green | Mims | 43.22 | |
| 2 | Alton | A | Singleton | Elliott-Barnes | Harris | Taul | 42.85 | |
| 3 | Madison | A | Moore | Boyd | Wilson Jr. | Bursey | 42.72 | |
| 4 | Andrew | A | Kelly | Ryan | Alexander | Michel | 42.52 | |
| 5 | Mt. Vernon | A | Lash | Hicks | Virden | Morrison | DNS | |
| 6 | Edwardsville | A | Hayes | Range | Valdez | Robinson | 42.75 | |
| 7 | Plainfield North | A | Gramley | Pauly | Brennan | Patterson | 43.14 | |
| 8 | Streator Township | A | Brown | White | Jeffries | Ford | 43.52 |