| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Damon Fowler | Webster | 09.20 | |
| 2 | john smith | Lincoln College | 08.73 | |
| 3 | Lawrence McNairy | Missouri Baptist | 08.51 | Illinois College Snow Bird Open |
| 4 | Christian Herring | Lindenwood-Belleville | 08.24 | |
| 5 | Jonah Peoples | Greenville | 08.35 | Principia College Prin Relays |
| 6 | Andrew Pieper | Columbia (Mo.) | 08.64 | 2016 Jayhawk Classic |
| 7 | Larry Stewart | Belhaven | 09.05 | UAB Vulcan Invitational |
| 8 | Sean Kisch | Illinois College | 09.23 | Illinois College Snow Bird Open |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | Sammy Salinas | Illinois College | 10.86 | Illinois College Snow Bird Open |
| 4 | Bryan Hodges | Lindenwood-Belleville | 10.11 | Principia College Prin Relays |
| 5 | Andrae Griffin | Belhaven | NT | |
| 6 | Matt Kole | Unattached | 10.00 | |
| 7 | Richard James | Missouri Baptist | 09.42 | Principia College Prin Relays |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | daniel welling | Lincoln College | 09.25 | |
| 4 | Michael Casebolt | Hannibal-LaGrange | 09.24 | |
| 5 | deonta johnson | Lincoln College | 09.25 | |
| 6 | Dustin Wintjen | Culver-Stockton | 09.32 | IWU Top Times Titan Open |
| 7 | David Jackson | Maryville (Mo.) | 10.00 |