| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Knox | A | 3:30.00 | |
| 2 | Culver-Stockton | A | 3:30.00 | |
| 3 | Belhaven | A | 3:30.00 | |
| 4 | Lincoln College | A | 3:27.50 | |
| 5 | Lindenwood-Belleville | A | 3:22.50 | |
| 6 | Missouri Baptist | A | 3:24.83 | Central Methodist Invite |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Greenville | B | 3:36.50 | |
| 2 | Knox | B | 3:34.20 | |
| 3 | Lindenwood-Belleville | B | 3:33.00 | |
| 4 | Hannibal-LaGrange | A | 3:32.00 | |
| 5 | Greenville | A | 3:32.00 | |
| 6 | Illinois College | A | 3:32.00 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Lindenwood-Belleville | C | NT | |
| 3 | Maryville (Mo.) | A | 3:45.95 | 2016 John Craft Invitational |
| 4 | Illinois Track Club | A | 3:44.00 | |
| 5 | Culver-Stockton | B | 3:37.00 | |
| 6 | William Woods | A | 3:37.00 |